To measure cell viability, 74 µl/well CyQUANT ® NF DNA binding dye was added to the cells and the cells were incubated for 60 min at 37 °C before fluorescence was measured using 485 nm excitation and 530 nm emission using a Spectramax M3 multi-mode microplate reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Description 1. SpectraMax Paradigm Multi-Mode Safety Guide 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The following document is intended to demonstrate setup of this instrument. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The optical density was read using a SpectraMax M3 (Molecular Devices, Sunnyvale, CA, USA). From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Our Price: $8,635. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax ABS Plus SpectraTest ABS2 v1. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 4/5 Set up plate template / Define Plate Map 1. ATP standard curves read on SpectraMax microplate readers. Guanidine hydrochloride at a final concentration of 5 M was used to denature a 10 mg/mL solution of lysozyme. 21. Spectral Range. . The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Select wells and then add or select a group or blank and assignThe SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Wavelength Selection. Multimode Readers Comparison Chart. ; The top 3 importers of Spectramax are India with 365. SpectraMax M3 Multi-Mode Microplate Reader. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. Add cells in appropriate medium to microplate wells. 0 out of 5 $ 11,999. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. D 47 7. Sphere-like cells were subcultured into single-cell suspension, and cells of ≥90% viability were used for sphere formation studies. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. A. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. . MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. 314 low lamp level warning. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. gingivalis -treated BMDMs (MOI = 50), with or without pretreatment of OTTSP167 or Compound 50 for 2 h. . Case Study 1 — Molecules Showed High Conformational Similarity: In the first case study, we analyzed multiple batches of innovator Herceptin (trastuzumab) and biosimilar MAbs using the antibody-array ELISA. Software Manual – SoftMax Pro. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. SpectraMax Glo and DuoLuc. Tested w/warranty. Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper Module. For crystal violet. DCFH-DA and JC-1 were used to detect intracellular ROS and mitochondrial membrane potential. Unparalleled performance on a personalized platform. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] i3x Multi-Mode Detection Platform Features User-configurable applications User-configurable detection modules expand the reader’s detection capabilities to. . It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. 1 & 7. SpectraMaxi3Multi. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forIntroduction. SpectraMax® M3. . 1. If filters are used with the SpectraMax iD5 reader, integration time may be set to 50 ms, reducing the. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Instruments: Cary 630 FTIR, SpectraMax® M3 Multi-Mode Microplate Reader, AR 2000 and TA. Please contact Tatiana to reserve the plate reader time: tnl1@psu. The chaperone function of PfHsp70-1 under various experimental conditions. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. 1) SpectraMax Glo Steady Luc Reporter Assay (6. Offer Ends In: 5 Days 05: 02: 03. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. No. Spectranet customer portalPage 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. {"product":{"total":4,"offset":0,"limit":4,"data":[{"path":"/products/microplate-readers/multi-mode-readers/spectramax-m2-m2e-readers","identifier":"SpectraMax M2/M2e. no. 2. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Wait 20 seconds. 더 많은. SpectraMax ® M2 Multi-Mode Microplate Reader. Use the 2. When a SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. SpectraMax® Aquamax. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. Resolution: The validation protocols are included in the v5. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. . I am looking to meet/connect with people from all walks of life. Finally, the absorbance was measured with a SpectraMax M3 microplate reader (Molecular Devices Corporation, Sunnyvale, CA, USA) at a wavelength of 450 nm. 2. Actual shake speed is based on the microplate format. Description 1. The drawing shows a standard microplate of 15 mm height covered with a lid. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 37) was ordered from Sigma Aldrich (St. The following document is intended to demonstrate setup of this instrument. Luminescent signals were measured with a Spectramax M3 multimode plate reader over a 1 s integration time. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Our absorbance plate readers feature our PathCheck Sensor. Molecular Devices VersaMax Microplate Reader. Make sure that the microplate reader feet are completely seated in the mounting cups. 标准功. The SpectraMax M2 and M2e are versatile and reliable. ROS detection. SpectraMax® M4. Luciferase Detection Instrumentation Guide. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. Luciferase Detection Instrumentation Guide. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. SpectraTest ABS1 Absorbance Validation Plate. Shake intensity. The SpectraTest ABS1 Validation Plate package. No accessories are needed and SoftMax® Pro. Introduction. Serum IL-8 level quantification Serum IL-8 levels were quantified in sera from patients after HSCT using an enzyme-linked immunosorbent assay (Cat. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forBioz Stars score, Techniques, Protocol Conditions and more for Spectramax M3 Spectrophotometer, supplied by Danaher Inc. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. For all. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The innovative and flexible design features. For cell viability assay, cells were incubated for 4 h with 1 mg/ml resazurin solution (10 μl), and then the fluorescence intensity was measured using a SpectraMax M3 microplate reader (Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 544 nm and emission wavelength of 595 nm . 313 reference gain check fail. Choose from a three- (M3), four- (M4), or SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Call 516-939-0888 to check current. Read mode. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Learn how to use the SpectraTest ABS2 Validation Plate, a convenient and reliable tool for verifying the performance of your absorbance microplate reader. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. spr SpectraMax iD5 SpectraTest ABS2 v1. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10 The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. 305 reference level saturation. Next to the pushbutton is a green LED. spr SpectraMax Plus 384. 1) Select the correct plate and the wells that you want to measure. When a feature or capability applies to only certain readers, this exception is noted. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. User Manual – SpectraMax M3 M4 M5 M5e. The team have compared the effects of antibiotics on bacterial viability and. book Page 1 Wednesday, July 7, 2010 2:09 PM Plate chamber design of the SpectraMax category 1 microplate readers. Instructions for using SIRT1 siRNA and scrambled siRNA were provided by Santa Cruz Biotechnology (CA, USA). Microplate Reader Shelf. Learn how you can utilize microplate readers to drive landmark discoveries. book Page 1 Wednesday, July 7, 2010 2:09 PM. . Using the PathCheck® Sensor, results can be automatically normalized to a 1 cm pathlength so that the values are. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. Other technologies such as imaging. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. Mitosox works fine in 96 wells. Summary of Contents for Molecular Devices SpectraMax M2. The % parasite viability, as indicated by the pLDH activity in the treated wells compared with the untreated controls, was calculated. 1step assay with SpectraMax microplate readers. spr * SpectraMax i3(x) SpectraTest ABS2 v1. SpectraMax Line of Readers Now Includes 14 Unique Configurations to Accommodate Wide Range of Customer Detection Needs MDS Analytical Technologies Launches Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate ReadersAutomated Functions Autofocus, autoLED intensity, auto-exposure Autofocus Method Image-based autofocus User-trained autofocus Laser autofocus (option)2. The absorbance was measured at 230. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Access this by going to Help >. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. Qualify the fluorescence performance of the SpectraMax . Example: [Drawer]. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. 1. 72 MB. Choose from a three- (M3), four- (M4), orThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Page 12 SpectraMax MiniMax Imaging Cytometer Installation Guide 10. ZERO BIAS - scores, article reviews, protocol conditions and moreInstruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. Recertification is recommended every two years. 4. All associated StakMax® stackers. 2. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. Multi-Mode Microplate Readers. 0200-6117. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Finally, SpectraMax M3 microplate reader was used to detect 412 nm absorption values, which were positively correlated with GSH levels. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Polyplex sizes and zeta potentials were measured with a Malvern Instruments Zetasizer (Nano-2590) at a scattering angle of 173°. Then, 25 nM HOPS complex, 50 nM Sec18, 600 nM Sec17, and finally 100 nM Vam7 were added. FlexStation™ II. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Antiretroviral efficacy was determined by measuring HIV reverse transcriptase (RT) activity in culture medium. D 1 1. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Click template editor icon in Home panel or select configure your plate layout in plate setup helper a. The staphyloxanthin intermediates present in collected supernatant were quantified by measuring at different wavelengths using multi-label reader (Spectramax M3, USA). SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. For performing enzyme assays SpectraMax M3 Multi-Mode Microplate Reader (Molecular device, USA) was used. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. e. These lab workhorses are used in drug discovery, research, bioassay validation, and. AquaMax® 4000. The result was expressed as mg gallic acid. In the M2, M5 and Plus 384, the light. SpectraMax Spectrofluorometers. 0 mm hex key. The guide also covers the software features, the specifications, and the accessories of the systems. . Fluorescence was measured using a SpectraMax M3 plate reader (excit/emiss = 480/520 nm). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. e. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Summary: Accurate and reproducible A260 measurements can be made easily in SpectraMax Microplate Spectrophotometers with awareness of the optical properties of microplate materials and attention to technique. The DNA extracts were quantified using Quanti-iT™ Picogreen ® dsDNA reagent (Invitrogen, Paisley UK) on a black 96 well plate at an absorbance of 520 nm. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. spr SpectraMax iD3 SpectraTest ABS2 v1. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. The supernatant was collected after 20 min of incubation in the dark. The absorbance was assessed at 405 nm by a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. XTplusC Texture Analyser Languages: French, English and Greek. Operated fromTo ensure loading of equal amounts of protein for each sample, colorimetric protein quantification was performed using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) and a SpectraMax M3 spectrophotometer (Molecular Devices, Sunnyvale, CA). Expand your lab’s capabilities to include flash applications such as dual luciferase and. Expanded wavelength coverage. Finally, the absorbance was measured with a SpectraMax M3 microplate reader (Molecular Devices Corporation, Sunnyvale, CA, USA) at a wavelength of 450 nm. 00 . Description 1. The integration time suggested is 5-10 sec. S. Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. The exactly same condition without CCK-8 solution was used to subtract background signal that is absorbed by cell medium or natural product. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Description 1. Examples: “Press carefully to engage,” “ Do not press down. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. A purple product is formed when pLDH is present , and this product is quantified in a Spectramax M3 microplate reader at the absorbance of 620 nm. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Mitochondrial activity was expressed by the decrease in OD (450–660 nm), discounting the average of the ODs of the controls . • Tel. 1) EarlyTox Glutathione Assay (6. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The shift in emission peak is also automaticallyThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Fluorescent Polarization: Solid black plates, as above. Transfection with siRNA. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. On the front panel of the instrument, press the load/eject button to move the microplate drawer outside of the instrument. Using a SpectraMax M3 spectrophotometer from Molecular Devices, we measured the color change at 450 nm. 9000-0756. 4 kilograms). Detection modalities are shown in Table 1-1. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. book Page 1 Wednesday, July 7, 2010 2:09 PMA SpectraMax ® M3 microplate reader luminometer was used to quantify the chemiluminescence from the reactions as relative luminescence units (RLUs) every four seconds for three minutes. 0112-0112F 3 Contents Chapter1:SpectraTestABS1ValidationPlateOverview 5 CertificateofCalibration 6 ISO-17025 6 AvailableTests 7 Chapter2:ValidationProtocols 9SpectraMax® L (software version 5. book Page 1 Wednesday, July 7, 2010 2:09 PM. SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. . Find many great new & used options and get the best deals for Replacement Bulb for Molecular Devices SpectraMax M3 Flash Lamp at the best online prices at eBay! Free shipping for many products! Skip to main content. For more sample throughput, the system can StakMax Handling Stacker Base Unit. Cells were cultured for at least 10 days in sphere-forming medium. Acarbose was used as a. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. SoftMaxProSoftwareUserGuide 2 5014177G ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevices,LLC(“Molecular Devices”)equipment,software,reagents. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Accuris SmartDrop. In the SoftMax Pro Software, select the Operation tab and click Change Aperture to display a message. Select wells and then add or select a group or blank and assignA microplate reader that can detect two or more applications is considered a multi-mode plate reader. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence. DevicesThe SpectraMax M2 And M2e Systems Provide The Multi Detection Microplate Readers With Dualmonochromators, Dual-mode Cuvette Ports, With Top & Bottom Reading. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Photometric accuracy. The net area under the curve (AUC) was estimated by subtracting the AUC of the blank from the AUC of the reacted. HistologyWe have been using a SpectraMax M3 for several years and have found it excellent, but more recently have been looking to use assays which require Time Resolved Fluorescence (TRF) operation (really. For more detailed information and technical support of Life Technologies assays including specific conditions for assay windows between 2-3 fold, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Microplate Readers Biotek Synergy 2 Plate. Note: In this user guide, all references to SpectraMax Multi-Mode. 315 can't find zero order. AquaMax® 2000. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. D 1 1. 314 low lamp level warning. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. For more information, please visit us at per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. INTRODUCTION: Proper physiological function relies on the vascular system to distribute oxygenating blood to all tissues, return deoxygenated blood to the lungs, and maintain tissue homeostasis, including functions such as hemostasis, lipid transport, and immune surveillance. The fluorescence of the reaction was read every 3 min (λ excitation = 485 nm, λ emission = 538 nm) for up to 90 min at constant 37 °C with the SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). For example, microplates containing different samp les can be read using the same orCell viability는 세포막 Integrity 또는. The plate was placed into the SpectraMax L Luminometer and a 1-second integrated read taken of each selected well using the MycoAlert protocol in the SoftMax ® Pro Software (Read A). For standard curve values, an albumin standard (Thermo Fisher Scientific) dilution serious. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Wavelength Range. 4. 7 mm for all shake intensities. SpectraMax® ABS Plus. Figure 1. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Hydrophobicity assayThese settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. SpectraMax Luminometer Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3. Subsequently, the PBS solutions containing the cells and the MSNs were incubated at 37°C under shaking at 220 rpm for another 4 h, after which the PBS supernatants were transferred into the wells of a 96-well plate and evaluated on a fluorescent spectrophotometer (SpectraMax M3, Molecular Devices, USA) using an. Immunofluorescence ASC antibody (1:200, Abcam, ab155970, UK) was used for immunofluorescence in P. SpectraMax Paradigm Multi-Mode Detection Platform User Guide Loading and Unloading Microplates To load or unload a microplate: 1. Bioz Stars score: 96/100, based on 1 PubMed citations. The TPC of the samples was calculated by plotting a standard curve with different concentrations of gallic acid (10, 30, 60, 100, and 200 mg/L). Keywords: Spectramax M2 Systems, Spectramax M2e Systems, Spectramax M2/m2e Readers, Pathcheck Sensor, Spectratest Abs1 Absor Jun 8th, 2023SpectraMax M3, M4,. Page 28 Chapter 5: Read Modes and Read Types 1. Moffitt Cancer Center. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the barriers to novel, landmark discoveries. Find regional support. 1. Microtitre plates were incubated for 2 h at 37°C in the dark. Other technologies such as imaging. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Cell Degranulation. SpectraMax M3, M4, M5, and M5 e. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. M5 reader microplate m3 spectramax molecular devices llc m5e biocompare quote fluorescenceMolecular devices spectramax plus 384 user manual pdf download Spectramax® m2/m2e multimode microplate readers, with softmax® proMolecular devices spectramax m5 microplate reader labx contract svc warranty available manufacturer. Figure 6: Transcreener ADP2 FI assay principle. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 160 多种预存实验方法可以直接调用，可以进行数据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功能，可以方便的进行各种数 据结果的展示。Popular answers (1) Sanjay Premi. Recommended SpectraMax iD3/iD5 reader settings. SpectraMax® Microplate Readers and SoftMax® Pro Software. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. 1 release preventing the protocols from being used for validation. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is a multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Page 22 StakMax Microplate Handling System User Guide 3. . It does not work as expected. To determine growth rate, the log phase of growth. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Concisely, digested test composite was treated with NaNO 2 (5%) and incubated for 5 min. 0 out of 5 $ 2,999. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentThe SpectraMax iD3 reader offers the sensitivity and wavelength scanning capability needed to perform intrinsic tryptophan fluorescence measurements. Twenty five μL of supernatant was removed from each sample and placed in a 96-well clear bottom plate and read at 546/575 nm (excitation/emission) using a SpectraMax M3 fluorescent microplate reader (Molecular Devices, Sunnyvale, CA). Also, when using a SpectraMax M4 Reader,. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. The entire SpectraMax M range includes a cuvette port for Absorbance, Fluorescence, and from SpectraMax M3 also for Luminescence. Page 13 Installing and Setting Up the. Bioz Stars score: 86/100, based on 1 PubMed citations. This process should take about 3 - 5 minutes. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and.